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Ellen Jorgensen: What you need to know about CRISPR
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Should we bring back the wooly mammoth? Or edit a human embryo? Or wipe out an entire species that we consider harmful? The genome-editing technology CRISPR has made extraordinary questions like these legitimate -- but how does it work? Scientist and community lab advocate Ellen Jorgensen is on a mission to explain the myths and realities of CRISPR, hype-free, to the non-scientists among us.
Ellen Jorgensen - Biologist, community science advocate
Ellen Jorgensen is at the leading edge of the do-it-yourself biotechnology movement, bringing scientific exploration and understanding to the public. Full bio
Ellen Jorgensen is at the leading edge of the do-it-yourself biotechnology movement, bringing scientific exploration and understanding to the public. Full bio
Double-click the English transcript below to play the video.
00:12
So, has everybody heard of CRISPR?
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I would be shocked if you hadn't.
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This is a technology --
it's for genome editing --
it's for genome editing --
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and it's so versatile and so controversial
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that it's sparking all sorts
of really interesting conversations.
of really interesting conversations.
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Should we bring back the woolly mammoth?
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Should we edit a human embryo?
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And my personal favorite:
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How can we justify
wiping out an entire species
wiping out an entire species
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that we consider harmful to humans
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off the face of the Earth,
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using this technology?
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This type of science
is moving much faster
is moving much faster
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than the regulatory mechanisms
that govern it.
that govern it.
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And so, for the past six years,
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I've made it my personal mission
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to make sure that as many people
as possible understand
as possible understand
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these types of technologies
and their implications.
and their implications.
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Now, CRISPR has been the subject
of a huge media hype,
of a huge media hype,
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and the words that are used most often
are "easy" and "cheap."
are "easy" and "cheap."
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So what I want to do is drill down
a little bit deeper
a little bit deeper
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and look into some of the myths
and the realities around CRISPR.
and the realities around CRISPR.
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If you're trying to CRISPR a genome,
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the first thing that you have to do
is damage the DNA.
is damage the DNA.
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The damage comes in the form
of a double-strand break
of a double-strand break
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through the double helix.
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And then the cellular repair
processes kick in,
processes kick in,
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and then we convince
those repair processes
those repair processes
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to make the edit that we want,
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and not a natural edit.
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That's how it works.
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It's a two-part system.
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You've got a Cas9 protein
and something called a guide RNA.
and something called a guide RNA.
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I like to think of it as a guided missile.
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So the Cas9 --
I love to anthropomorphize --
I love to anthropomorphize --
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so the Cas9 is kind of this Pac-Man thing
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that wants to chew DNA,
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and the guide RNA is the leash
that's keeping it out of the genome
that's keeping it out of the genome
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until it finds the exact spot
where it matches.
where it matches.
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And the combination of those two
is called CRISPR.
is called CRISPR.
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It's a system that we stole
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from an ancient, ancient
bacterial immune system.
bacterial immune system.
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The part that's amazing about it
is that the guide RNA,
is that the guide RNA,
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only 20 letters of it,
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are what target the system.
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This is really easy to design,
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and it's really cheap to buy.
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So that's the part
that is modular in the system;
that is modular in the system;
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everything else stays the same.
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This makes it a remarkably easy
and powerful system to use.
and powerful system to use.
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The guide RNA and the Cas9
protein complex together
protein complex together
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go bouncing along the genome,
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and when they find a spot
where the guide RNA matches,
where the guide RNA matches,
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then it inserts between the two strands
of the double helix,
of the double helix,
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it rips them apart,
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that triggers the Cas9 protein to cut,
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and all of a sudden,
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you've got a cell that's in total panic
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because now it's got a piece
of DNA that's broken.
of DNA that's broken.
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What does it do?
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It calls its first responders.
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There are two major repair pathways.
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The first just takes the DNA
and shoves the two pieces back together.
and shoves the two pieces back together.
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This isn't a very efficient system,
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because what happens is
sometimes a base drops out
sometimes a base drops out
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or a base is added.
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It's an OK way to maybe, like,
knock out a gene,
knock out a gene,
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but it's not the way that we really want
to do genome editing.
to do genome editing.
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The second repair pathway
is a lot more interesting.
is a lot more interesting.
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In this repair pathway,
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it takes a homologous piece of DNA.
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And now mind you, in a diploid
organism like people,
organism like people,
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we've got one copy of our genome
from our mom and one from our dad,
from our mom and one from our dad,
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so if one gets damaged,
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it can use the other
chromosome to repair it.
chromosome to repair it.
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So that's where this comes from.
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The repair is made,
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and now the genome is safe again.
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The way that we can hijack this
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is we can feed it a false piece of DNA,
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a piece that has homology on both ends
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but is different in the middle.
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So now, you can put
whatever you want in the center
whatever you want in the center
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and the cell gets fooled.
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So you can change a letter,
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you can take letters out,
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but most importantly,
you can stuff new DNA in,
you can stuff new DNA in,
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kind of like a Trojan horse.
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CRISPR is going to be amazing,
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in terms of the number of different
scientific advances
scientific advances
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that it's going to catalyze.
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The thing that's special about it
is this modular targeting system.
is this modular targeting system.
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I mean, we've been shoving DNA
into organisms for years, right?
into organisms for years, right?
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But because of the modular
targeting system,
targeting system,
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we can actually put it
exactly where we want it.
exactly where we want it.
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The thing is that there's
a lot of talk about it being cheap
a lot of talk about it being cheap
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and it being easy.
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And I run a community lab.
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I'm starting to get emails from people
that say stuff like,
that say stuff like,
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"Hey, can I come to your open night
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and, like, maybe use CRISPR
and engineer my genome?"
and engineer my genome?"
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(Laugher)
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Like, seriously.
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I'm, "No, you can't."
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(Laughter)
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"But I've heard it's cheap.
I've heard it's easy."
I've heard it's easy."
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We're going to explore that a little bit.
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So, how cheap is it?
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Yeah, it is cheap in comparison.
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It's going to take the cost of the average
materials for an experiment
materials for an experiment
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from thousands of dollars
to hundreds of dollars,
to hundreds of dollars,
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and it cuts the time a lot, too.
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It can cut it from weeks to days.
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That's great.
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You still need a professional lab
to do the work in;
to do the work in;
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you're not going to do anything meaningful
outside of a professional lab.
outside of a professional lab.
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I mean, don't listen to anyone who says
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you can do this sort of stuff
on your kitchen table.
on your kitchen table.
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It's really not easy
to do this kind of work.
to do this kind of work.
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Not to mention,
there's a patent battle going on,
there's a patent battle going on,
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so even if you do invent something,
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the Broad Institute and UC Berkeley
are in this incredible patent battle.
are in this incredible patent battle.
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It's really fascinating
to watch it happen,
to watch it happen,
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because they're accusing each other
of fraudulent claims
of fraudulent claims
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and then they've got people saying,
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"Oh, well, I signed
my notebook here or there."
my notebook here or there."
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This isn't going to be settled for years.
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And when it is,
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you can bet you're going to pay someone
a really hefty licensing fee
a really hefty licensing fee
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in order to use this stuff.
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So, is it really cheap?
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Well, it's cheap if you're doing
basic research and you've got a lab.
basic research and you've got a lab.
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How about easy?
Let's look at that claim.
Let's look at that claim.
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The devil is always in the details.
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We don't really know
that much about cells.
that much about cells.
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They're still kind of black boxes.
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For example, we don't know
why some guide RNAs work really well
why some guide RNAs work really well
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and some guide RNAs don't.
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We don't know why some cells
want to do one repair pathway
want to do one repair pathway
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and some cells would rather do the other.
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And besides that,
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there's the whole problem
of getting the system into the cell
of getting the system into the cell
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in the first place.
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In a petri dish, that's not that hard,
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but if you're trying to do it
on a whole organism,
on a whole organism,
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it gets really tricky.
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It's OK if you use something
like blood or bone marrow --
like blood or bone marrow --
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those are the targets
of a lot of research now.
of a lot of research now.
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There was a great story
of some little girl
of some little girl
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who they saved from leukemia
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by taking the blood out, editing it,
and putting it back
and putting it back
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with a precursor of CRISPR.
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And this is a line of research
that people are going to do.
that people are going to do.
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But right now, if you want to get
into the whole body,
into the whole body,
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you're probably going
to have to use a virus.
to have to use a virus.
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So you take the virus,
you put the CRISPR into it,
you put the CRISPR into it,
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you let the virus infect the cell.
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But now you've got this virus in there,
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and we don't know what the long-term
effects of that are.
effects of that are.
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Plus, CRISPR has some off-target effects,
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a very small percentage,
but they're still there.
but they're still there.
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What's going to happen
over time with that?
over time with that?
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These are not trivial questions,
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and there are scientists
that are trying to solve them,
that are trying to solve them,
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and they will eventually,
hopefully, be solved.
hopefully, be solved.
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But it ain't plug-and-play,
not by a long shot.
not by a long shot.
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So: Is it really easy?
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Well, if you spend a few years
working it out in your particular system,
working it out in your particular system,
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yes, it is.
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Now the other thing is,
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we don't really know that much about how
to make a particular thing happen
to make a particular thing happen
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by changing particular spots
in the genome.
in the genome.
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We're a long way away from figuring out
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how to give a pig wings, for example.
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Or even an extra leg -- I'd settle
for an extra leg.
for an extra leg.
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That would be kind of cool, right?
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But what is happening
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is that CRISPR is being used
by thousands and thousands of scientists
by thousands and thousands of scientists
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to do really, really important work,
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like making better models
of diseases in animals, for example,
of diseases in animals, for example,
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or for taking pathways
that produce valuable chemicals
that produce valuable chemicals
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and getting them into industrial
production and fermentation vats,
production and fermentation vats,
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or even doing really basic research
on what genes do.
on what genes do.
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This is the story of CRISPR
we should be telling,
we should be telling,
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and I don't like it
that the flashier aspects of it
that the flashier aspects of it
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are drowning all of this out.
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Lots of scientists did a lot of work
to make CRISPR happen,
to make CRISPR happen,
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and what's interesting to me
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is that these scientists
are being supported by our society.
are being supported by our society.
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Think about it.
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We've got an infrastructure that allows
a certain percentage of people
a certain percentage of people
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to spend all their time doing research.
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That makes us all the inventors of CRISPR,
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and I would say that makes us all
the shepherds of CRISPR.
the shepherds of CRISPR.
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We all have a responsibility.
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So I would urge you to really learn
about these types of technologies,
about these types of technologies,
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because, really, only in that way
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are we going to be able to guide
the development of these technologies,
the development of these technologies,
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the use of these technologies
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and make sure that, in the end,
it's a positive outcome --
it's a positive outcome --
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for both the planet and for us.
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Thanks.
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(Applause)
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ABOUT THE SPEAKER
Ellen Jorgensen - Biologist, community science advocateEllen Jorgensen is at the leading edge of the do-it-yourself biotechnology movement, bringing scientific exploration and understanding to the public.
Why you should listen
In 2009, after many years of working as a molecular biologist in the biotech industry, together with TED Fellow Oliver Medvedik, Jorgensen founded Genspace, a nonprofit community laboratory dedicated to promoting citizen science and access to biotechnology. Despite criticism that bioresearch should be left to the experts, the Brooklyn-based lab continues to thrive, providing educational outreach, cultural events and a platform for science innovation at the grassroots level. At the lab, amateur and professional scientists conduct award-winning research on projects as diverse as identifying microbes that live in Earth's atmosphere and (Jorgensen's own pet project) DNA-barcoding plants, to distinguish between species that look alike but may not be closely related evolutionarily. Fast Company magazine named Genspace one of the world's "Top 10 innovative companies in education."
Ellen Jorgensen | Speaker | TED.com